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CH 10-13 Miscellaneous Terms

AB
Coombs reagentAn anti-human immunoglobulin reagent used to enhance the detection of immunoglobulin G (IgG) antibodies in blood bank testing.
Endpoint methodA radial immunodiffusion technique in which antigen is allowed to diffuse out until the point of equivalence is reached.
HemagglutinationAn antigen–antibody reaction that results in the clumping of red blood cells (RBCs).
FluorophoresCompounds that absorb energy from an incident light source and convert that energy into light of a longer wavelength and lower energy as the excited electrons return to the ground state.
ImmunohistochemistryThe use of labeled antibodies to directly detect tumor markers in tissue
Western blotA confirmatory test that has been used for some infections (e.g., HIV, Lyme disease) based on separation of antigens from the pathogen by electrophoresis followed by transfer or blotting of the antigen pattern to a supporting medium for reaction with test serum.
HybridizationSpecific binding of two single-stranded DNA segments, as in binding of a probe with a known nucleic acid sequence to an unknown piece of DNA
GenomeThe entire set of genes in an organism.
Dual-parameter dot plotGrouping of cells based on two different characteristics, one of which is plotted on the x-axis and the other on the y-axis
Single-parameter histogramPlot of a chosen parameter or measurement on the x-axis against the number of events on the y-axis.
ImmunophenotypingIdentifying cells according to their surface antigen expression.
Intrinsic parametersight-scattering properties that are a part of the cell, such as size and granularity



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