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MLS Lab Ops

AB
Converting mg/dL to SI Units (mmol/L) Multiply mg/dL by 10 to get mg/Lthen divide by atomic weight in mg/mmol. Formula: (mg/dL × 10) / Atomic Weight.
Converting mEq/L to mg/dL First calculate milliequivalent weight (Atomic Weight / Valence)multiply by mEq/L to get mg/L
Dilution Formula (C1V1 = C2V2) C1 × V1 = C2 × V2where C1 = stock concentration
pH Formula for a Strong Acid pH = -log[H+]. Example: If [H+] = 2.5 × 10^-2 MpH = -log(0.025) = 1.6.
pH Formula for a Strong Base Calculate pOH = -log[OH-]then pH = 14 - pOH. Example: If [OH-] = 1.5 × 10^-5 M
Significant Figures Rules for Zeros Zeros to the left of non-zero digits are not significant (e.g.0.060 M has 2 sig figs). Trailing zeros after a decimal point are significant.
Beer-Lambert Law Equation Cu = (Au / As) × Cswhere Cu = concentration of unknown
Gaussian Distribution QC Confidence Limits 68.3% of values fall within ±1s95.4% within ±2s
Random Error (RE) Definition & Causes Unpredictable error affecting precision. Caused by bubblestemperature spikes
Systematic Error (SE) Definition & Causes Predictable error affecting accuracy in one direction. Caused by reagent decaybad calibration
Individualized Quality Control Plan (IQCP) CLIA QC option based on risk management. Evaluates risk of testing error across preanalyticalanalytical
Linear Regression Equation (ŷ = mx + b) ŷ = mx + b; where slope (m) represents proportional errorand y-intercept (b) represents constant error in method evaluation.
Total Analytical Error (TE) Formula Total Error = Bias + 2s (or Bias + 1.96s)combining both systematic bias and random variability to evaluate CLIA acceptability.



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