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DNA Technology

AB
Genetic engineeringThe application of molecular genetics for practical purposes.
DNA technologyThe technology involved in genetic engineering.
Restriction enzymesBacterial enzymes used to cut DNA molecules into more manageable pieces.
Sticky endsWhen a restriction enzyme cuts DNA and single-chain "tails" are formed.
Blunt endsWhen a restriction enzyme cuts DNA and no single-chain "tails" are formed.
Cloning vectorA carrier that is used to clone a gene and transfer it from one organism to another.
PlasmidA ring of DNA found in a bacterium in addition to its main chromosome.
Donor geneA specific gene isolated from another organism.
Gene cloneAn exact copy of a gene.
InsulinA protein that controls sugar metabolism.
Genomic libraryThe set of thousands of DNA pieces from a genome.
Recombinant DNAThe combination of DNA from two or more sources.
Transgenic organismA host receiving recombinant DNA.
DNA fingerprintA pattern of bands made up of specific fragments from an individual's DNA.
RFLP analysisExtracting DNA from a specimen of blood or other tissue and cutting it into fragments using restriction enzymes.
Gel electrophoresisThe separation of nucleic acids or proteins, primarily according to their size and charge.
ProbesRadioactive segments of DNA that are complementary to the segments being compared.
Polymerase chain reaction (PCR)Often used to make many copies of selected segments of the available DNA in order to conduct further investigations.
PrimerAn artifically made single-stranded sequence of DNA required for the initiation of replication.
Human Genome ProjectThe determination of the entire human genome.
Gene therapyTreating a genetic disorder by introducing a gene into a cell or by correcting a gene defect in a cell's genome.

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