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DNA and Gel electrophoresis terms

AB
DNAdeoxyribonucleic acid, is a very large molecule called a polymer
Polymermeans molecule with many units
nucleotidesunits or monomers of DNA
Nucleotides 2made up of a 5-carbon sugar (deoxyribose) a phosphate group and one of four nitrogen bases
Aadenine
Gguanine
Ccytosine
Tthymin
Purine BasesAdenine and guanine and have a double ring structure
Pyrimidine BasesCytosine and thymine and have a single ring structure
Deoxyribose Sugarsugar of the DNA backbone has 3 oxygen and 5 carbon atoms
DNA2composed of twisting strands of nucleotides
A and Talways join together and form two hydrogen bonds
C and Galways join together and form three hydrogen bonds
complementarythe two strands of DNA (AT na CG)
DNA Fingerprintinga method of identification that compares fragments of DNA
DNA3the genetic material found within the cell nucleus
DNA fingerprintconstructed by first obtaining a DNA sample from body tissue or fluid
Gel electrophoresisa method that separates macromolecules like DNA on the basis of size, electric charge, and other physical propertie
gelsimilar to Jell-O in consistency
Electrorefers to the energy of electricity
Phoresisfrom the Greek verb phoros, means "to carry across
Holescreated in the gel that serves as a reservoir to hold the DNA solution
DNA solutions(mixtures of different sizes of DNA fragments) are loaded into a well in the gel
gel matrixacts as a sieve for DNA molecules
Large moleculesmolecules have difficulty getting through the holes in the matrix
Small moleculesmove easily through the holes
DNA fingerprinting technologyhas helped scientists to discover the genetic causes of many disease processes
DNA fingerprinting 2helped advance forensic science and paternity testing



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