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Step 3: This step is very similar to the conversion of pyruvate to acetyl-CoA in the transition phase. It involves a large multienzyme complex called the alpha-ketoglutarate dehydrogenase complex. The end result is that co-enzyme A is added to our molecule as carbon dioxide is removed to form a 4 carbon molecule called succinyl CoA.

Succinyl CoA helps regulate the alpha-ketoglutarate dehydrogenase complex when supplies of succinyl CoA are high. The excess succinyl CoA acts as an inhibitor, providing feedback that slows the Kreb's Cycle as needed.

 

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