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Glycolysis

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Step 5: In this step, each molecule of PGAL gives up a hydrogen and two high energy electrons to the hydrogen accepting molecule known as NAD+. The resulting molecule, NADH, will carry these high energy electrons to the electron transport chain which you will learn about later on. The reaction described above is sufficiently exothermic that the energy released can drive an endothermic reaction in which inorganic phosphate from the cytoplasm can be added to our PGAL molecule to form 1,3 biphosphoglyeric acid. The enzyme responsible is glyceraldehyde-3-phosphate dehydrogenenase.

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